Shimadzu Spd20a User Manual Site

Navigate to the system maintenance menu on the keypad or LabSolutions software to reset the lamp usage hour meter back to zero. Flow Cell Cleaning and Flushing

190 nm to 700 nm (up to 900 nm on the SPD-20AV). Wavelength Accuracy: ±1plus or minus 1 Noise Level: AU (under specified standard conditions). Drift:

Ensure the detector is placed on a stable surface, away from direct sunlight, air conditioning drafts, and significant vibrations to maintain baseline stability. 3. Operation Procedures and Methods

Warm-up: The D2 lamp requires at least 30 minutes to stabilize before starting an analysis. Operating before the lamp is warm can result in baseline drift. Operating Procedures shimadzu spd20a user manual

Press the zero or autozero button to establish a clean baseline before initiating your injection run. Dual-Wavelength Mode

The SPD-20A uses an internal low-pressure mercury lamp for automatic wavelength calibration in the UV region.

: Capable of monitoring two different wavelengths simultaneously, which is essential for identifying peaks and ensuring purity. Sensitivity : Features a noise level of and a drift of , ensuring reliable data even at low concentrations. Navigate to the system maintenance menu on the

Allow the detector to warm up for at least 30-60 minutes to ensure baseline stability.

Connect the PEEK or stainless steel tubing coming from the column outlet to the "IN" port of the flow cell. Use a standard 1/16-inch fingertight fitting.

is recognized for its industry-leading sensitivity and stability. Deuterium (D2) lamp. Wavelength Range: 190 to 700 nm. Noise Level: Drift: Ensure the detector is placed on a

Before powering the unit, the operator must ensure the physical integrity of the system. The initialization process begins with the flow path. The manual dictates the importance of priming the system with the mobile phase to remove air bubbles, which can scatter light and cause significant baseline noise. Once the fluid path is established, the lamp should be ignited. The SPD-20A utilizes a deuterium (D2) lamp for UV detection and an optional tungsten lamp for the visible range. A critical operational note found in the user manual is the warm-up period; the lamp requires approximately 30 to 60 minutes to stabilize. Attempting to run an analysis before thermal equilibrium is reached will result in baseline drift and unreliable quantification.

Check power cord connections. Inspect and replace the rear panel fuses if blown. Lamp has exceeded lifespan or connection is loose. Check the lamp plug connection. Replace the D2cap D sub 2 lamp if it is burnt out. High Baseline Noise Air bubbles in flow cell, or aging lamp.